"Academic Staff" (male / female / non-binary) in the research project "Metabolic Distinction of Apoptosis-Induced Compensatory Proliferation and Apoptosis-Induced Apoptosis", MetaLife, founded by the German Research Foundation (DFG)

Furtwangen University is offering a position to the earliest possible date in the Medical & Life Sciences department as "Academic Staff" (male / female / non-binary) in the research project "Metabolic Distinction of Apoptosis-Induced Compensatory Proliferation and Apoptosis-Induced Apoptosis", MetaLife, founded by the German Research Foundation (DFG) as a fixed-term contract limited to 36 months. Working hours per week will be 25,675 hours (65 %). Employment conditions are regulated by the German TV-L regulations (max. E13 TV-L), depending on responsibilities and qualifications of the individual candidate. The position is located at our Villingen-Schwenningen campus. We do expect you to be prepared to undertake tasks at the other campuses, too, however. Your tasks will include: The position is part of a DFG-funded project that focuses on the identification of metabolic signalling molecules released by dying cells. The project builds on recent findings that apoptotic cells secrete antiinflammatory metabolites that promote wound healing (see e. g. here DOI: 10.1038/s-3). The new project focuses on the question of which biologically active metabolites are secreted during different forms of cell death (e. g. apoptosis or necroptosis) and what effects these have on other cells. An activity-based approach will be employed to identify secretomes that induce an equal or opposite effect in the target cells and subsequently identify the responsible metabolites using high-resolution ion mobility coupled mass spectrometry. For this purpose, state-of-the-art methods such as cell cultivation and cell-cell signalling in microfluidic systems, multiparameter flow cytometry and ion mobility coupled mass spectrometry are used. The tasks of the vacant position include the establishment of (primary) cell models for different types of cell death and their corresponding transfer to microfluidic systems, the establishment and application of multiparameter flow cytometry methods to verify the different types of cell death and to identify the effects on the target cells, the analytical characterization of metabolic products using established methods based on liquid chromatography-coupled high-resolution mass spectrometry, the mechanistic investigation of the identified substances and the bioinformatic evaluation of the various data sets. Collaboration in project management (e. g. reporting, presentation of project results) as well as participation in the daily laboratory organization are also expected. The candidate is expected to write a doctoral thesis on the project results. We are looking for: A scientific university degree (Diploma or Master's degree with a grade of at least 2.5) with a focus on biology, cell biology, molecular biology, biochemistry, bioanalytics, biotechnology, pharmacy, molecular medicine or related subjects. Experience in the cultivation of human cells and classical molecular biology methods (e. g. MTT assay, caspase activity assay) are required. Experience in flow cytometry, microfluidics, liquid chromatography (e. g. HPLC, UHPLC), extraction methods for metabolites from biological samples, bioinformatic tools such as R or Phyton, mass spectrometry or statistical analysis of metabolomics datasets is an advantage. Good written and spoken English and German language skills are also expected. Wir bieten: You will find this a stimulating position with diverse tasks, working as part of a motivated and open team and enjoying the numerous benefits offered by a position within the German civil service system. Furtwangen University is an equal opportunities employer and is committed to increasing the diversity of its faculty. As we seek to increase the proportion of women lecturers and researchers, we encourage female candidates to apply. Disabled candidates with suitable qualifications are given preferential consideration. You will find detailed information on our university online at . For additional information on this position, please contact Prof. Dr. Hans-Peter Deigner: phone . or email . We look forward to your application. Please submit it via our Online Application Tool at no later than 03.11.2024, citing the code 2024-080-MLS-D.